教員紹介

SDGsへの取り組み

最終学歴

岡山大学大学院 自然科学研究科博士後期課程　修了

取得学位

博士(薬学)

所属学会

日本細菌学会、日本薬学会、毒素シンポジウム

現在の専門分野

微生物学、細菌学、分子生物学

現在の研究課題

●病原性エロモナスのプロテアーゼの産生調節機構に関する研究
●環境微生物の多様性、および環境適応に関する研究

主要研究業績

●病原性エロモナスの菌体外タンパク質の性状解析に関する研究
●インド・コルカタ市における臨床由来および環境水由来コレラ菌の性状比較解析

最近の研究業績

1. S. Seike, H. Kobayashi, M. Ueda, E. Takahashi, K. Okamoto, H. Yamanaka, Outer membrane vesicles released from Aeromonas strains are involved in the biofilm formation, Front. Microbiol., 2021, 11, 613650

2. D. Morita, M. Morita, M. Alam, A. K. Mukhopadhyay, F. Johura, M. Sultana, S. Monira, N. Ahmed, G. Chowdhury, S. Dutta, T. Ramamurthy, P. Samanta, E. Takahashi, K. Okamoto, H. Izumiya, M. Ohnishi, Whole-genome analysis of clinical Vibrio cholerae O1 in Kolkata, India and Dhaka, Bangladesh reveals two lineages of circulating strains indicating variation in genomic attributes, mBio, 2020, 11(6), e01227-20

3. S.S. Paul, E. Takahashi (co-first author), G. Chowdhury, S. Miyoshi, T. Mizuno, A.K. Mukhopadhyay, S. Dutta, K. Okamoto, Low viability of cholera toxin-producing Vibrio cholerae O1 in t in the artificial low ionic strength aquatic solution, Biol. Pharm. Bull., 2020, 43(8), 1288-1291

4. D. Morita, E. Takahashi, M. Morita, M. Ohnishi, T. Mizuno, S. Miyoshi, D. Dutta, T. Ramamurthy, G. Chowdhury, A.K. Mukhopadhyay, K. Okamoto, Genomic characterization of antibiotic resistance-encoding genes in clinical isolates of Vibrio cholerae non-O1/non-O139 strains from Kolkata, India; Generation of novel types of genomic islands containing plural antibiotic resistance genes, Microbiol. Immunol., 2020, 64(6), 435-4443

5. O. Yamasaki, E. Takahashi, K. Noda, N. Kanaya, S. Tanabe, Y. Shirakawa, S. Morizane, Surgical site infection caused by Aeromonas hydrophila presenting as necrotizing soft tissue infection after esophagectomy, J. Dermatol., 2020, 47(6), 673-676

6. H. Kobayashi, S. Seike, M. Yamaguchi, M. Ueda, E. Takahashi, K. Okamoto, H. Yamanaka, Aeromonas sobria serine protease decreases epithelial barrier function in T84 cells and accelerates bacterial translocation across the T84 monolayer in vitro, PLoS One, 2019, 14(8), e0221344

7. G. Chowdhury, T. Ramamurthy, A. Ghosh, S. Dutta, E. Takahashi, A.K. Mukhopadhyay, Emergence of Azithromycin Resistance Mediated by Phosphotransferase -Encoding mph(A) in Diarrheagenic Vibrio fluvialis, mSphere, 2019, 4(3), e00215-19

8. Y. Yamada, S. Watanabe, K. Okamoto, S. Arimoto, E. Takahashi, K. Negishi, T. Negishi, Chloroethylating anticancer drug-induced mutagenesis and its repair in Escherichia coli, Genes Environ., 2019, 41, 11

9. H. Kobayashi, T. Otsubo, F. Teraoka, K. Ikeda, S. Seike, E. Takahashi, K. Okamoto, T. Yoshida, H. Tsuge, H. Yamanaka, Involvement of the Arg566 residue of Aeromonas sobria serine protease in substrate specificity, PLoS One, 2017, 12(10), e0186392

10. K. Nakano, Y. Yamada, E. Takahashi, S. Arimoto, K. Okamoto, K. Negishi, T. Negishi, E. coli mismatch repair enhances AT-to-GC mutagenesis caused by alkylating agents, Mutat. Res., 2017, 815, 22-27

11. E. Takahashi, C. Fujinami, T. Kuroda, Y. Takeuchi, S. Miyoshi, S. Arimoto, T. Negishi, K. Okamoto, Indolo[3,2-b]quinoline derivatives suppressed the hemolytic activity of beta-pore forming toxins, aerolysin-like hemolysin produced by Aeromonas sobria and alpha-hemolysin produced by Staphylococcus aureus, Biol. Pharm. Bull., 2016, 39(1), 114-120

12. E. Takahashi, H. Ozaki, Y. Fujii, H. Kobayashi, H. Yamanaka, S. Arimoto, T. Negishi, K. Okamoto, Properties of hemolysin and protease produced by Aeromonas trota, PLoS One, 2014, 9(3), e91149

13. X. Fang, T. Nunoshiba, M. Yoshida, A. Nishikawa, K. Nemoto, M. Degawa, S. Arimoto, K. Okamoto, E. Takahashi, T. Negishi, Effects of Oral Administration of Non-genotoxic Hepato-hypertrophic Compounds on Metabolic Potency of Rat Liver, Genes Environ., 2014, 36(1), 1-9

14. E. Takahashi, S. Oono, S. Yamamoto, S. Arimoto, T. Negishi, K. Okamoto, Spermidine-analogous triamines suppressed the growth of Candida albicans., Biol. Pharm. Bull., 2013, 36(9), 1440-1447

15. E. Takahashi, H. Kobayashi, H. Yamanaka, M. Nakanishi, A. Tateishi, T. Abe, S. Arimoto, T. Negishi, K. Okamoto, Analysis of carboxy terminal domain of metalloprotease of elastolytic Aeromonas hydrophila, Biol. Pharm. Bull., 2013, 36(7), 1174-1182

16. K. Taira, S. Kaneto, K. Nakano, S. Watanabe, E. Takahashi, S. Arimoto, K. Okamoto, R.M. Schaaper, K. Negishi, T. Negishi, Distinct pathways for repairing mutagenic lesions induced by methylating and ethylating agents, Mutagenesis, 2013, Feb 27, 1-10

17. E. Takahashi, H. Ito, H. Kobayashi, H. Yamanaka, Y. Takeda, G.B. Nair, S. Arimoto, T. Negishi, K. Okamoto , Production and properties of lipase of Aeromonas sobria, Microbiol. Immunol., 2012, 56(5), 295-307

18. T. Kurakawa, H. Kubota, H. Tsuji, K. Matsuda, T. Asahara, T. Takahashi, T. Ramamurthy, T. Hamabata, E. Takahashi, S. Miyoshi, K. Okamoto, A.K. Mukhopadhyay, Y. Takeda, K. Nomoto, Development of a sensitive rRNA-targeted reverse transcription-quantitative polymerase chain reaction for detection of Vibrio cholerae/mimicus, V.
parahaemolyticus/alginolyticus and Campylobacter jejuni/coli., Microbiol. Immunol., 2012, 56(1), 10-20

19. L.M. Bao, A. Nozaki, E. Takahashi, K. Okamoto, H. Ito, T. Hatano, Hydrolysable tannins isolated from Syzygium aromaticum: structure of a new c-glucosidic ellagitannin and spectral features of tannins with a tergalloyl group, Heterocycles, 2012, 85 (2), 365-381

20. K. Yoneda, A. Shiozawa, A. Kitahara, E. Takahashi, S. Arimoto, K. Okamoto, T. Negishi, Novel antimutagenic proteins in the edible mushroom Agrocybe cylindrasea, Genes Environ., 2012, 34(1), 9-17

21. E. Takahashi, Y. Fujii, H. Kobayashi, H. Yamanaka, G.B. Nair, Y. Takeda, S. Arimoto, T. Negishi, K. Okamoto, Inhibition of biosynthesis of metalloprotease of Aeromonas sobria by sodium chloride in the medium, Microbiol. Immunol., 2011, 55 (1), 60-65

22. E. Takahashi, H. Kobayashi, H. Yamanaka, G.B. Nair, Y. Takeda, S. Arimoto, T. Negishi, K. Okamoto, Maturation pathway of metalloprotease produced by Aeromonas sobria, Microbiol. Immunol., 2010, 54 (10), 596-605

23. 野崎阿季子, 髙橋栄造, 岡本敬の介, 伊東秀之, 波多野力, Candida 属真菌に対する各種精油および精油成分の抗真菌作用，およびAmphotericin Bの抗真菌作用に及ぼす影響, YAKUGAKU ZASSHI, 2010, 130 (6), 895-902

24. H. Kobayashi, A. Tateishi, H. Tsuge, E. Takahashi, K. Okamoto, H. Yamanaka, The carboxy-terminal tail of Aeromonas sobria serine protease is associated with the chaperone, Microbiol. Immunol., 2009, 53(12), 647-657

25. 山中 文, 波多野 力, 伊藤秀之, 谷口抄子, 髙橋栄造, 岡本敬の介, グァバ飲料およびグァバ葉に含まれるタンニンおよび関連ポリフェノールの食中毒細菌に及ぼす抗菌作用, 機能性食品と薬理栄養, 2009, 5(4), 217-222

26. R. Khan, E. Takahashi, H. Nakura, M. Ansaruzzaman, S. Banik, T. Ramamurthy, K. Okamoto, Toxin production by Aeromonas sobria in natural environments: river water vs. seawater, Acta Med. Okayama, 2008, 62 (6), 363-371

27. H. Yamanaka, H. Kobayashi, E. Takahashi, K. Okamoto, MacAB is involved in the secretion of Escherichia coli heat-stable enterotoxin, J. Bacteriol., 2008, 190 (23), 7693-7698

28. F. Yamanaka, T. Hatano, H. Ito, S. Taniguchi, E. Takahashi, K. Okamoto, Antibacterial effects of guava tannnins and related polyphenols on Vibrio and Aeromonas species, Nat. Prod. Commun., 2008, 3(5), 711-720

29. K. Namba, X. Zheng, K. Motoshima, H. Kobayashi, A. Tai, E. Takahashi, K. Sasaki, K. Okamoto, H. Kakuta, Design and synthesis of benzenesulfonanilides active against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus., Bioorg. Med. Chem., 2008, 16, 6131-6144

30. E. Takahashi, Z. Sultan, S. Shimada, W.W. Aung, M.M. Nyein, K. N. Oo, G.B. Nair, Y. Takeda, K. Okamoto, Studies on the diarrheagenic Escherichia coli isolated from children with diarrhea in Myanmar, Microbiol. Immunol., 2008, 52(1), 1-7

31. Y. Fujii, K. Tsurumi, M. Sato, E. Takahashi, K. Okamoto, Fluid secretion caused by aerolysin-like hemolysin of Aeromonas sobria in the intestines is due to stimulation of production of prostaglandin E2 via cyclooxygenase-2 by the intestinal cells., Infect. Immun., 2008, 76(3), 1076-1082

32. R. Khan, E. Takahashi, T. Ramamurthy, Y. Takeda, K. Okamoto, Salt in surroundings influences the production of serine protease into milieu by Aeromonas sobria., Microbiol. Immunol., 2007, 51(10), 963-976

33. H. Yamanaka, S. Tadokoro, M. Miyano, E. Takahashi, H. Kobayashi, K. Okamoto, Studies on the region involved in the transport activity of Escherichia coli TolC by chimeric protein analysis., Microb. Pathog., 2007, 42(5-6), 184-192

34. E. Takahashi, S. Arimoto, K. Okamoto, T. Negishi, Enhancement of phase II enzyme activity by purpurin resulting in the suppression of MeIQx-DNA-adduct formation in mice., Mutat. Res., 2007, 626(1-2), 128-134

著書及び総説

著書

1. 岡本敬の介，髙橋栄造, エロモナス科　エロモナス属, 病原細菌・ウイルス図鑑 (新居志郎（代表）・倉田毅・林英生・本田武司・小田紘・松本明編集委員), p200-203, 2017年, 北海道大学出版会

2. 髙橋栄造, 第6章 病気と遺伝子工学 6.1 遺伝子変異とは, 遺伝子工学 -基礎から医療まで- (監修 早津彦哉, 編集　根岸和雄、中西徹), p189-195, 2017年, 廣川書店

3. 髙橋栄造, エロモナス感染症, 化学療法の領域「いわゆる“ヒト喰いバクテリア”と劇症感染症」 2013年6月号, p1478-1481, 医薬ジャーナル社

4. E. Takahashi, K. Okamoto, ASP peptidase, Handbook of Proteolytic Enzymes (Third Ed)(Eds, Neil D. Rawlings and Guy S. Salvesen), Chapter 706: 3209-3213, 2013., Elsevier

5. E. Takahashi, Keinosuke Okamoto, PA peptidase (Aeromonas), Handbook of Proteolytic Enzymes (Third Ed)(Eds, Neil D. Rawlings and Guy S. Salvesen), Chapter 118: 577-579, 2013., Elsevier

6. S. Miyoshi, K. Okamoto, E. Takahashi, Vibriosin, Handbook of Proteolytic Enzymes (Third Ed)(Eds, Neil D. Rawlings and Guy S. Salvesen), Chapter 119: 579-582, 2013., Elsevier